Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a . Schematic of the tagged ENaCγ subunit produced from the ENaCγ-VF allele, which includes a C-terminal mVenus, 3C protease site, and 3xFLAG tag. b . Representative immunofluorescence image showing co-localization of mVenus and ENaCγ in kidney tissue. mVenus signal was detected using a FITC-conjugated anti-GFP primary antibody (goat, Rockland, 1:100) and Alexa Fluor 488 donkey anti-goat secondary antibody. Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody. DAPI is 4’,6-diamidino-2-phenylindole . c . Close-up view highlighting localization of tagged ENaCγ.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Produced, Immunofluorescence

Journal: bioRxiv

Article Title: Differential Assembly of Native ENaC Complexes Across Mouse Epithelial Tissues

doi: 10.64898/2026.01.23.701393

Figure Lengend Snippet: a. Cryo-EM map of human ENaC in complex with two Fabs, 10D4 and 7B1, shown in side and top-down views. In the top-down view, a red box highlights the interaction site between 7B1 and the α subunit. b. Sequence alignment of the experimentally determined 7B1 epitope region in human and mouse ENaCα subunits, revealing high conservation and supporting the potential for cross-reactivity with mouse ENaC. c . Schematic representation of the 7B1-mScarlet Fab construct used to detect the ENaC α subunit. The variable regions of the 7B1 heavy and light chains were expressed as a Fab fragment in HEK293 cells, with mScarlet fused to the C-terminus of the heavy chain. d . FSEC trace monitoring mVenus fluorescence from lung lysates of ENaCγ-VF mice, showing the elution profile of γ-containing ENaC complexes. Dotted lines indicate the elution range for mVenus-tagged complexes (11-16 mL). e . FSEC trace from the same sample following 7B1-mScarlet fluorescence, revealing the elution profile of α-containing ENaC complexes. Dashed lines show that the α-associated signal is restricted to a narrower elution range (11-13 mL), suggesting that α-γ co-assemblies represent a more defined subset of the total γ-containing complexes. f . Overlay of the FSEC traces shown in panels d and e , plotted on the same axes to facilitate direct comparison of elution profiles and peak positions. The inset shows the full normalized traces for each condition. The main panel displays a magnified view of the gray-shaded region indicated in the inset, highlighting the relative alignment of the major peaks.

Article Snippet: Endogenous ENaCγ was detected using a rabbit anti-ENaCγ primary antibody (StressMarq, 1:500) targeting the C-terminus, and Alexa Fluor 647 donkey anti-rabbit secondary antibody.

Techniques: Cryo-EM Sample Prep, Sequencing, Construct, Fluorescence, Comparison

Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Expression of 3 hub genes (A) Gene expression of KCNK1, PCDH1 and SDR16C5. (B) Expression of KCNK1 in a single cell. (C) Expression of PCDH1 in a single cell. (D) Expression of SDR16C5 in a single cell.

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing, Gene Expression

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Related assays on the expression of hub genes. (A–C) mRNA expression of KCNK1, PCDH1 and SDR16C5 in HPNE and BXPC3. (D) mRNA expression of PCDH1 in HPNE and multiple PDAC cell lines. (E) Matched pairs of PDAC tissues and adjacent non-neoplastic pancreatic tissues were stained with PCDH1-specific antibodies for immunohistochemical analysis. Representative images of tissues and tumor-infiltrating cells are shown (100×, scale bar = 100 μm; 400×, scale bar = 20 μm). The MOD of PCDH1 is obtained by analyzing the photo-optical density with IPP software. (F) ROC curve of PCDH1 in PDAC. (G) Protein-protein interaction network of PCDH1. (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing, Staining, Immunohistochemical staining, Software

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Correlation between PCDH1 and immune infiltration. (A) Correlation between PCDH1 and immune score. (B) Correlation between PCDH1 and stromal score. (C) Correlation between PCDH1 and ESTIMATE score. (D) Correlation between PCDH1 and immune cells. (* P < 0.05, ** P < 0.01).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques:

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Chemosensitivity of PCDH1. (A) Correlation between GDSC drug sensitivity and PCDH1 mRNA expression (B) Correlation between CTRP drug sensitivity and PCDH1 mRNA expression.

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the proliferation of PDAC. (A) Efficiency of knocking down mRNA of PCDH1 si-RNA in BXPC3. (B) Efficiency of knocking down mRNA of PCDH1 si-RNA in SW1990. (C, E) MTT assays reveal the proliferation ability of control group and PCDH1 knockdown group in cell line BXPC3 and SW1990. (D, F) Colony formation assays reveal the proliferation ability of control group and PCDH1 knockdown group in cell line BXPC3 and SW1990. (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Control

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the migration of PDAC. (A) Transwell assays show the migration ability of the control group and the PCDH1 knockdown group in the BXPC3 cell line. (Scale bar = 50μm) (B) Transwell assays show the migration ability of the control group and the PCDH1 knockdown group in the SW1990 cell line. (Scale bar = 50μm) (C) Wound healing assays exhibit the migration ability of the control group and the PCDH1 knockdown group in the BXPC3 cell line. (Scale bar = 100μm) (D) Wound healing assays exhibit the migration ability of the control group and the PCDH1 knockdown group in the SW1990 cell line. (Scale bar = 100μm) (ns: P >0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Migration, Control

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: Knockdown of PCDH1 inhibited the tumor stemness of PDAC cell lines. (A) Relative expression of CD24 mRNA in the control group and the PCDH1 knockdown group. (B) Relative expression of CD133 mRNA in the control group and the PCDH1 knockdown group. (C) Detection of CD24 + cells in the control and PCDH1 knockdown groups via flow cytometry in BXPC3 and SW1990 cell lines. (D) Detection of CD133 + cells in the control and PCDH1 knockdown groups via flow cytometry in BXPC3 and SW1990 cell lines. (E, F) Percentage of CD24 + cells between the control group and the PCDH1 knockdown group (G, H) Percentage of CD133 + cells between the control group and the PCDH1 knockdown group. (I, J) Sphere formation assay was performed to detect sphere formation ability of the control group and the PCDH1 knockdown group in BXPC3 and SW1990. (K, L) Diameter of tumor spheres at 24 h, 48 h and 5 days in the control group and the PCDH1 knockdown group.(Scale bar = 500μm) (ns: P >0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Knockdown, Expressing, Control, Flow Cytometry, Tube Formation Assay

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A) Heatmap of differential expression genes between the NC and si-PCDH1 groups. (B) KEGG enrichment analysis demonstrates pathways associated with differential expression genes. (C, D) Expression levels of mRNA for six molecules in the PI3K/Akt pathway were detected by qRT-PCR. (*** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, Quantitative Proteomics, Expressing, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A, B) MTT assay was used to detect the proliferation ability of the control, IGF-1, si-PCDH1, and si-PCDH1 + IGF-1 groups. (C–E) A colony formation assay was performed to detect the proliferation ability of the four groups. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, MTT Assay, Control, Colony Assay

Journal: Frontiers in Oncology

Article Title: PCDH1 facilitates migration, proliferation, and stemness of pancreatic cancer cells through PI3K-Akt signaling

doi: 10.3389/fonc.2025.1696695

Figure Lengend Snippet: PCDH1 promotes proliferation and migration of PDAC through activation of PI3K/Akt pathway. (A) Transwell assays present the migration capacity of the control, IGF-1, si-PCDH1, and si-PCDH1 + IGF-1 groups. (Scale bar = 100μm) (B, C) Counts of cell migration in four groups. (D, F) Wound healing assays exhibit the migration ability of the four groups. (Scale bar = 100μm) (E, G) Rate of cell migration in four groups. (** P < 0.01, *** P < 0.001, **** P < 0.00011).

Article Snippet: Slides were incubated overnight at 4 °C with a rabbit polyclonal anti-PCDH1 primary antibody (bs-111111R, Bioss Biotechnology, China) at the manufacturer-recommended dilution.

Techniques: Migration, Activation Assay, Control